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Image Search Results
Journal: PLoS ONE
Article Title: The Limonoids TS3 and Rubescin E Induce Apoptosis in Human Hepatoma Cell Lines and Interfere with NF-κB Signaling
doi: 10.1371/journal.pone.0160843
Figure Lengend Snippet: A: HepG2 cells were incubated with 10 μM TR3, TR4 or TR9 for 20 hours. Cell proliferation was measured by BrdU-Assay. DMSO control: 0.1%. B: HepG2 cells were incubated with 5 μM TR3, TR4 or TR9 for 6 hours. Expression of PCNA, p21 and HSA was measured by real time RT-PCR. DMSO control: 0.05%. ★ p ≤ 0.05, ★★★ p ≤ 0.001 vs DMSO controls. C + D: HepG2 cells were incubated with 5 μM TR3, TR4 or TR9 for 24 hours. Protein expression of Cyclin D1 was detected by Western Blot analysis (C) and quantified by using Image Lab TM Software (D). Quantification was performed in relation to reference expression of GAPDH as a loading control.
Article Snippet: Semiquantitative evaluations were performed using the VersaDoc TM Imaging System 4000 MP (Bio-Rad, Munich, Germany) followed by quantification using the
Techniques: Incubation, BrdU Staining, Control, Expressing, Quantitative RT-PCR, Western Blot, Software